Figure 4.

Brusatol (BT) downregulated the phosphorylation level of 4EBP1 and S6K1 in pituitary adenomas. (a) After 0–24 h treatment with BT (250 nM) in GH3 and MMQ cells, Western blot analysis was used to monitor the expression level of 4EBP1, S6K1, p-4EBP1, and p-S6K1. (b) Primary pituitary tumor cells (1 GHoma and 1 nonfunctional tumor) were treated with BT; Western blot analysis revealed the expression level of 4EBP1, S6K1, p-4EBP1, and p-S6K1. (c) Representative images of IHC-stained samples show that BT decreased p-4EBP1 and p-S6k1 expression in GH3 and MMQ xenograft models; scale bar, 100 μm.