Skip to main content
. 2021 Jun 16;2021:6696015. doi: 10.1155/2021/6696015

Figure 5.

Figure 5

Brusatol (BT) increased ROS accumulation in GH3 and MMQ cells by inhibiting the Nrf2 pathway. (a) GH3 and MMQ cells treated with BT (250 nM) for 24 h were analyzed by flow cytometry after DCFH-DA staining to determine ROS levels. (b) GH3 and MMQ cells were treated with a range of concentrations of BT for 24 h. Western blot analysis showed that BT downregulated the expression of Nrf2 and its downstream gene Ho-1 but had no effect on Keap1. (c) GH3 and MMQ cell lines were treated with BT (250 nM) and/or NAC (100 μM) for 24 h and cell viability measured by MTS assay. (d) GH3 and MMQ cell lines were treated with BT (250 nM) and/or NAC (100 μM) for 24 h; Western blot analysis showed that NAC reversed the inhibitory effect of BT on inhibition of p-4EBP1 and p-S6K1. Data are presented as the mean ± SD. ∗∗P < 0.01; ∗∗∗P < 0.001.