Fig. 1.

(a) Schematic illustration of the SWITCH/TDE clearing method. (b) 1 mm-thick slice of an adult human brain sample before and after the treatment. (c) Images of SYTOX Green labeled tissue at different depths. Scale bar = $100\mu \text{m}$. (d) Images of NeuN immunostained tissue at different depths. Scale bar = $50\mu \text{m}$. Acquisition obtained with TPFM. (e) Representative images of cleared tissues immunostained with various antibodies and DAPI ($4\mathrm{\prime },6$-Diamidino-2-Phenylindole, Dihydrochloride). Scale bar = $50\mu \text{m}$. Acronym list: Neuron-specific Nuclear protein (NeuN, all neurons), Microtubule-Associated Protein 2 (MAP2; pyramidal cells), Nonphosphorylated neurofilament protein (SMI32; pyramidal cells), Glutamic Acid Decarboxylase (GAD67; all GABAergic interneurons), Parvalbumin (PV; GABAergic interneurons subtype), Calbindin (CB; GABAergic interneurons subtype), Vasointestinal peptide (VIP; GABAergic interneurons subtype), Somatostatin (SST; GABAergic interneuron subtype), Neuropeptide Y (NPY; GABAergic interneuron subtype), Neurofilament (NF), Ionized calcium Binding Adaptor molecule 1 (Iba1; glial cells), Glial Fibrillary Acidic Protein (GFAP; glial cells), Glutamine synthetase (GluS), Vimentin (Vim; Microvasculature), Collagen IV (Coll IV; microvasculature).