Table 1. Table summarizing the dyes tested in this study. The P/M column denotes polyclonal vs monoclonal antibodies. The same abbreviations used in Fig. 1 are used. AF is a shorthand for Alexa Fluor.
Molecule | Company | Cat. n. | Host | P/M | Dilution |
---|---|---|---|---|---|
NeuN | Merck | ABN91 | Chicken | P | 1:50 |
GAD67 | Santa Cruz | sc-28376 | Mouse | M | 1:200 |
GAD65/67 | St John’s Lab | STJ93195 | Rabbit | P | 1:200 |
PV | Abcam | ab11427 | Rabbit | P | 1:200 |
PV | Abcam | ab32895 | Goat | P | 1:200 |
CB | Abcam | ab207528 | Rabbit | M | 1:200 |
VIP | Abcam | ab214244 | Rabbit | M | 1:200 |
SST | Abcam | ab30788 | Rat | M | 1:200 |
NPY | Abcam | ab6173 | Sheep | P | 1:200 |
NPY | Abcam | ab112473 | Mouse | M | 1:200 |
SMI-32 | Merck | NE1023 | Mouse | M | 1:200 |
Neurofilament | Abcam | ab4680 | Chicken | P | 1:200 |
GluS | Merck | MAB302 | Mouse | M | 1:200 |
MAP2 | Abcam | ab5392 | Chicken | P | 1:200 |
GFAP | Abcam | ab194324 | Rabbit | M | 1:200 |
Iba1 | Abcam | ab195031 | Rabbit | M | 1:200 |
Coll IV | Abcam | ab6586 | Rabbit | P | 1:200 |
Vim | Abcam | ab8069 | Mouse | M | 1:200 |
Anti-Rat IgG, AF 568 | Abcam | ab175475 | Donkey | P | 1:200 |
Anti-Rabbit IgG, AF 568 | Abcam | ab175470 | Donkey | P | 1:200 |
Anti-Chicken IgY, AF 568 | Abcam | ab175711 | Goat | P | 1:200 |
Anti-Mouse IgG, AF 568 | Abcam | ab175700 | Donkey | P | 1:200 |
Anti-Sheep IgG, AF 568 | Abcam | ab175712 | Donkey | P | 1:200 |
Anti-Rabbit IgG, AF 488 | Abcam | ab150077 | Goat | P | 1:200 |
Anti-Chicken IgY, AF 488 | Abcam | ab150169 | Goat | P | 1:200 |
DAPI | Thermo Fisher Scientific | D1306 | 1:1000 | ||
SYTOX Green | Thermo Fisher Scientific | S7020 | 1:1000 |