Skip to main content
. 2021 Mar 2;14(4):923–936. doi: 10.1038/s41385-021-00386-7

Fig. 1. Influence of C. rodentium infection on colonic ST2 and IL-33 expression and IL-33 production.

Fig. 1

a C57BL/6 mice were orally gavaged with CR on day 0. b Histopathological score of colon tissues assessed 10 days post infection. Bars represent the mean ± SEM of data from one representative experiment (n = 3 mice per group). c Colonic mRNA expression of the membrane-bound receptor ST2 measured at the indicated time points after infection by qRT-PCR. Bars represent fold change induction over d0 (n = 5–11 mice per group). d Serum sST2 concentrations determined on day 10 after infection via ELISA. e Il33 transcript levels (left panel) in colon biopsies and IL-33 protein secretion from in vitro cultured colon explants (right panel) were measured via RT-PCR and Luminex technology, respectively (n = 5–6 mice per group). f Secretion of IL-17A, TNF-α and IFN-γ from in vitro cultured colon explants determined by Luminex technology (n = 5–6 mice per group). All data are presented as mean ± SEM and were pooled from (c) three or (d, e, f) two independent experiments. Statistical analyses were performed using Mann–Whitney U test or Student’s t test. *P < 0.05; **P < 0.01; ***P < 0.001.