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. 2021 Feb 2;28(6):351–372. doi: 10.1038/s41434-021-00227-z

Fig. 3. Development of PDE6H MiniPromoters for cone cells.

Fig. 3

A Bioinformatic design of Ple348 and Ple349. ENCODE segments that potentially regulate the expression of the PDE6H gene are highlighted horizontally. Vertically highlighted genomic regions correspond to their color-matched segments included in the MiniPromoter design and are numbered as promoter(s) (P) and regulatory region(s) (RR). B Postnatal day 0 intravenous injection of Ple349-EmGFP, harvested 4 weeks later, led to robust expression in the outer nuclear layer (ONL). Scale bar, 100 µm. (For ubiquitous smCBA promoter see Fig. S1A.) C Adult intravitreal injection of Ple349-EmGFP, harvested 4 weeks later, led to robust expression in cone cells, as indicated by co-staining with the cone cell marker opsin, where EmGFP inner segments align to anti-opsin outer segments. Scale bar, 20 µm. (For smCBA see Fig. S1D.) EmGFP emerald green fluorescent protein, GCL ganglion cell layer, INL inner nuclear layer, IPL inner plexiform layer, OPL outer plexiform layer, TF transcription factor, TSS transcription start site. Green, anti-GFP; blue, Hoechst; red, anti-opsin.