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. 2021 Apr 28;594(7864):594–598. doi: 10.1038/s41586-021-03507-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a Pharmacology of wild-type GABA_B and the purification construct (EM) in a baclofen-mediated NanoBiT-G-protein dissociation assay. Data are mean ± s.e.m. from four independent experiments, performed in technical triplicate. b, Flow chart of the purification steps for the GABA_B–G_i1 complex. GABA_B was expressed in HEK293F cells. Heterotrimeric G_i1 and scFv16 were expressed in Hi5 cells. c–e, Size-exclusion chromatography profile (c), SDS–PAGE gel (d) and the negative-staining electron microscopy analysis (e) of the purified GABA_B–G_i1 complex.

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