Table 1.

Real-time PCR carried out using sense and antisense primers

Gene	Forward and reverse primer	Thermal cycles	Cycles N (bp)
AQP3	5′-CTCCAGCATCCGACAAGAAGC-3′ 5′-GAGGTCGTAGGCTGTTCTTCG-3′	5″ at 94 °C, 12″ at 58 °C, 28″ at 72 °C for 40 cycles	280
Integrin αV	5′-TAAGGCAGATGGCAAAGGAG-3′ 5′-CAGTGGAATGGAAACGATGAGC-3′	5″ at 94 °C, 10″ at 64 °C, 20″ at 72 °C for 40 cycles	510
Integrin β3	5′-GGTGCAATGAAGGGCGTGTTGG-3′ 5′--3′	5″ at 95 °C, 14″ at 57 °C, 26″ at 72 °C for 40 cycles	440
TNF-α	5′-CAGAGGGAAGAGTTCCCCAG-3′ 5′-CCTTGGTCTGGTAGGAGACG-3′	5″ at 95 °C, 6″ at 57 °C, 13″ at 72 °C for 40 cycles	324
IL-1α	5′-CATGTCAAATTTCACTGCTTCATCC-3′ 5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	5″ at 95 °C, 8″ at 55 °C, 17″ at 72 °C for 45 cycles	421
IL-1β	5′-GCATCCAGCTACGAATCTCC-3′ 5′-CCACATTCAGCACAGGACTC-3′	5″ at 95 °C, 14″ at 58 °C, 28″ at 72 °C for 40 cycles	708
TGF-β	5′-CCGACTACTACGCCAAGGAGGTCAC-3′ 5′-AGGCCGGTTCATGCCATGAATGGTG-3′	5″ at 94 °C, 9″ at 60 °C, 18″ at 72 °C for 40 cycles	439