KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-EPS8 (mouse) | BD Transduction Laboratories | Cat# 610144; RRID:AB_397545 |
| anti-GFP(chicken) | Aves Labs | Cat# GFP-1020; RRID:AB_10000240 |
| Goat anti-mouse Alexa Fluor 488 F(ab’)2 fragment | Molecular Probes | Cat# A-11017; RRID:AB_143160 |
| Goat anti-mouse Alexa Fluor 568 F(ab’)2 fragment | Molecular Probes | Cat# A11019; RRID:AB_143162 |
| Goat-anti chicken Alexa Fluor 488 IgG (H+L) | Molecular Probes | Cat# A-11039; RRID:AB_142924 |
| anti-Villin (rabbit) | Santa Cruz | Cat# sc28283; RRID: AB_2215971 |
| anti-Rabbit H+L Alexa Fluor 568 | Molecular Probes | Cat# A11011; RRID:AB_143157 |
| Bacterial and Virus Strains | ||
| E. coli DH5-Alpha competent cells | Molecular Cell Biology Resource Core, Vanderbilt Medical Center | Item# DH5 Alpha |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa-Fluor 568 Phalloidin | ThermoFisher | Cat# A12380 |
| Alexa-Fluor 488 Phalloidin | ThermoFisher | Cat# A12379 |
| ProLong Gold Antifade Reagent | Invitrogen | Cat# P36930 |
| 16% Paraformaldehyde solution | Electron Microscopy Sciences | Cat# 15710 |
| Triton X-100 | Sigma | Cat# T8787 |
| Lenti-X concentrator | Clontech | Cat# 631231 |
| FuGENE 6 | Promega | Cat# E2691 |
| Polybrene Infection Reagent | Sigma-Aldrich | Cat# TR-1003-G |
| Lipofectamine 2000 | ThermoFisher | Cat# 11668019 |
| Laminin | Corning | Cat#354232 |
| Phleomycin | InvivoGen | Cat# ant-ph-5 |
| Blasticidin | InvivoGen | Cat# ant-bl-1 |
| G418 Sulfate | Goldbio | Cat#G-418-25 |
| Doxycycline | RPI | Cat# 10592-13-9 |
| Cytochalasin D | Sigma | Cat# C2618 |
| CellMask Deep Red plasma membrane stain | Invitrogen | Cat# C10046 |
| Histoclear II | National Diagnostics | HS-202 |
| DMSO | Sigma | Cat# D8418 |
| Glutaraldehyde 25% | Electron Microscopy Sciences | Cat# 16220 |
| Tannic Acid | Electron Microscopy Sciences | Cat# 21700 |
| Osmium Tetroxide | Electron Microscopy Sciences | Cat# 19112 |
| Experimental Models: Cell Lines | ||
| Ls174T-W4 cells | Gift from Hans Clevers (Utrecht University, Netherlands) | RRID: CVCL J074 |
| HEK293FT | ATCC | CRL-3216 |
| HEK293T | ATCC | CRL-11268 |
| LLC-PK1-CL4 | Gift from Dr. Carolyn Slayman (Yale University) | N/A |
| CACO-2BBE | Gift from Dr. Mark Mooseker (Yale University) | N/A |
| OK | ATCC | CRL-1840 |
| Oligonucleotides | ||
| EGFP-EPS8 1-648 FORWARD: ATGAATGGTCATATTTCTAATCATCCC | This paper | N/A |
| EGFP-EPS8 1-648 REVERSE: TTATGACACAGGAACAGGTGCTGG | This paper | N/A |
| EGFP-EPS8 649-822 FORWARD: AAGGTCCCAGCAAATATAACACGTCA | This paper | N/A |
| EGFP-EPS8 649-822 REVERSE: TTAGTGACTGCTTCCTTCATCAAAAGATTCC | This paper | N/A |
| EGFP-EPS8 V690D L694D FORWARD: GAAATCTCAGATGGAGGAAGACCAAGATGAAGACATCCACAGACTGACCATTG | This paper | N/A |
| EGFP-EPS8 V690D L694D REVERSE: CAATGGTCAGTCTGTGGATGTCTTCATCTTGGTCTTCCTCCATCTGAGATTTC | This paper | N/A |
| EGFP-EPS8 LNK758-760AAA FORWARD: GGTGCACAACTTTTCTCTGCCGCTGCGGATGAACTGAGGACAGTCTGCCC | This paper | N/A |
| EGFP-EPS8 LNK758-760AAA REVERSE: GGGCAGACTGTCCTCAGTTCATCCGCAGCGGCAGAGAAAAGTTGTGCACC | This paper | N/A |
| MCHERRY-BACTIN FORWARD: TAAGCAGAATTCGCCACCATGGTGAGCAAGG | This paper | N/A |
| MCHERRY-BACTIN REVERSE: TAAGCAGGGCCCCTAGAAGCATTTGCGGTGGACGA | This paper | N/A |
| Recombinant DNA | ||
| pEGFP-C1-EPS8 | 23, 28 | N/A |
| pEGFP-EPS8 1-648 | 23 | N/A |
| pEGFP-EPS8 649-822 | This paper | N/A |
| pEGFP-EPS8 V690D L694D | This paper | N/A |
| pEGFP-EPS8 LNK758-760AAA | This paper | N/A |
| pLVX-mCherry-βactin | This paper | N/A |
| pLVX-mCherry-Espin | This paper | N/A |
| pmCherry-Espin | Gift from Dr. James Bartles, NWU | N/A |
| pEzrin-EGFP | 46 | Addgene #20680 |
| pEGFP-C1-IRTKS | 23, 28 | N/A |
| psPAX2 | Dr. Didier Trono | Addgene #12260 |
| pMD2.G envelope plasmid | Dr. Didier Trono | Addgene #12259 |
| Software and Algorithms | ||
| NIS Elements AR Analysis | Nikon Instruments | https://www.nikoninstruments.com/Products/Software |
| Prism10 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| FIJI/ ImageJ | Open source software | http://imagej.net/Fiji/Downloads |
| Other | ||
| 35 mm glass bottom dishes | CellVis | D35-20-1.5-N |