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. 2021 Jun 23;11:13186. doi: 10.1038/s41598-021-92574-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of hypoxia and/or heat treatment on resilience pathways-related proteins in the co-culture model. Relative protein expression of HSP-70 (A), HSF-1 (C), HIF-1α (D) and cytoplasmic (E) and nuclear Nrf2 (F) assessed by Western Blot. All target proteins were normalized to reference protein β-actin (total/cytoplasmic) or histone H3 (nuclear). (B) HSP-70 mRNA levels assessed by qRT-PCR. The target genes were normalized with housekeeping gene β-actin. All values were presented as means ± SD (N = 3, n = 3). Statistical differences were analyzed by two-way ANOVA followed by the Bonferroni’s multiple comparison test. Means without a common letter differ at p < 0.05.