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. 2021 Jun 23;11:13186. doi: 10.1038/s41598-021-92574-5

Figure 4.

Figure 4

Effects of hypoxia and/or heat treatment on TJ/AJ proteins in the co-culture model. Relative protein expression of ZO-1 (A), occludin (B), claudin-3 (C) and E-cadherin (D) assessed by Western Blot. All target proteins were normalized to reference protein β-actin. ZO-1 (E), occludin (F), claudin-3 (G) and E-cadherin (H) mRNA levels were assessed by qRT-PCR. The target genes were normalized to housekeeping gene β-actin. All values were presented as means ± SD (N = 3, n = 3). Statistical differences were analyzed by two-way ANOVA followed by the Bonferroni’s multiple comparison test. Means without a common letter differ at p < 0.05. OCLD occludin; CLDN3 claudin-3; E-cad E-cadherin.