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. 2021 Jun 23;11:13186. doi: 10.1038/s41598-021-92574-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of hypoxia and/or heat treatment on TJ localization in the co-culture model. Red or green color: anti-ZO-1, anti-OCLD or anti-CLDN3 antibody conjugated with Alexa-Fluor fluorescent secondary antibodies. The images were acquired by Leica TCS SP8 microscope with HCX IRAPO L 25 × /0.95 objective lens at 1.6 × digital magnification, pinhole: 1.5 AU. OCLD occludin; CLDN3 claudin-3.