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. 2021 Jun 23;6:88. doi: 10.1038/s41541-021-00344-1

Fig. 1. Proof-of-concept for a cell-based assay providing a functional readout for TT.

Fig. 1

a Assay layout. b Anti-TT IgG-specific enzymatic activity response in an assay performed with PBMC obtained from 11 donors before and after DTaP booster vaccination. Paired samples from individual donors before and after vaccination were tested in parallel. The graph summarizes the results from n = 3 independent experiments performed with 6, 4, and 1 donors, respectively. Bar graphs represents the interquartile range with median and whiskers depicting maximal and minimal values, each dot represents mean of enzymatic activity of a single donor. Data were analyzed using two-tailed Wilcoxon matched-pairs signed rank test (black lines and symbols), Mann–Whitney test (blue line and symbols). c ELISpot wells coated with TT for detection of anti-TT IgG of one representative donor showed (Donor #2). d Anti-TT IgG enzymatic activity of 14 buffy coat donors in response to CpG + adsorbed TT compared to the response to CpG + adsorbed DT or to CpG + aluminum adjuvant. Bar graphs represent the interquartile range with median and whiskers depicting maximal and minimal value, each dot represents mean of enzymatic activity of a single donor. Combined values from 14 buffy coats (n = 4 independent experiments) donors with statistical evaluation (Wilcoxon matched-pairs rank test). P values are depicted in the respective graphs, ns non-significant. The dotted line shows anti-TT IgG enzymatic activity of 200, the threshold of reactivity.