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. 2021 Jun 23;6:88. doi: 10.1038/s41541-021-00344-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Overview of the results from two consecutive rounds of experiments in 101 and 41 donors, respectively. b Means of enzymatic activity of anti-TT IgG produced in response to CpG + TT from 30 donors in first and second round of experiment (Donors are arranged according increasing mean value of anti-TT IgG enzymatic activity in a first round of experiments). Bar graphs represent mean (+ standard deviation) of all technical replicates (four and eight technical replicates per donor cell sample in first and second experiment, respectively). First round of experiments: n = 101 independent donors and n = 11 independent experiments. Second round of experiments: n = 41 independent donors and n = 9 independent experiments. c Spearman correlation of means of enzymatic activity anti-TT IgG response to CpG + TT from first and second round of experiment (30 reactive donors).