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. 2021 Jun 23;11:13099. doi: 10.1038/s41598-021-92473-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Almost all transplanted hemocytes develop into GCs. Hemocytes were isolated from donor crayfish, labeled with a nontoxic fluorescence cell tracer (green) and transplanted into recipients. Hemocytes were taken from the recipients and analyzed at different time points. The percentages of fluorescent GCs and SGCs in the total hemocytes were determined by flow cytometry and fluorescence microscopy. Over 10,000 cells were analyzed for each sample (A). Five animals were used in each group. Data from different individuals are indicated by different symbols. Images of transplanted cells are shown (B). Fluorescent SGCs are indicated with arrows, and fluorescent GCs are indicated with arrowheads. Bar, 25 μm.