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. 2021 Jun 23;11:13099. doi: 10.1038/s41598-021-92473-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The development of transplanted cells can be divided into three stages. In the transplantation experiments, the differentiation and maturation of hemocytes could be roughly separated into three morphological stages (Stage-1, -2, -3) based on light microscopy observations (A). Stage-1, early hemocytes with no/some small granules; Stage-2, cells with some medium size granules; Stage-3, mature cells filled with many large granules. The changes in the proportions of cells in these three stages during the developmental process are shown: (B) HPT cell transplantation; (C) total hemocyte transplantation; (D) SGC transplantation. Bar, 25 μm.