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. 2021 Jun 23;4:771. doi: 10.1038/s42003-021-02304-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The procedure of off-target analysis in HEK293T cells. 330 ng of genomic DNA corresponding to 5.0 × 10⁴ cells. b Off-target candidate sites and their mutation rate. The mutation rate was calculated by the following formula: [(total reads − no variants)/total reads]. For the on-target site, reads with in-frame 16–19 CAG repeats were summed up as no variants.