Table 1.

Data collection and refinement statistics.

Data collection	Lysozyme
Energy (eV)	12,659
Expose time (ms)	100
Space group	P43212
Cell dimension (Å)
a	79.45
b	79.45
c	38.47
Collected images	36,450
Hits images	11,958
Indexed images	7288
Indexed pattern	7423
Resolution (Å)	80.0–1.65 (1.71–1.65)
Unique reflections	15,398 (1498)
Completeness (%)	100.0 (99.93)
Multiplicity	83.7 (57.7)
SNR	6.58 (1.58)
CC	0.9836 (0.5635)
CC*	0.9958 (0.8490)
Rsplit (%)a	10.69 (70.26)
Wilson B factor (Å2)	31.76
Refinement
Resolution (Å)	56.18–1.65 (1.70–1.65)
Rworkb	17.85 (27.74)
Rfreec	20.87 (27.92)
R.m.s. deviations
Bond length (Å)	0.013
Bond angle (°)	1.618
B factors (Å)
Protein	31.23
Water	34.03
Ramachandran (%)
Preferred	98.43
Allowed	1.57
Outliers	0.00

Highest resolution shell is shown in parentheses.

^aR_split = $\left(\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$\sqrt{2}$}\right.\right)·\frac{{\sum }_{\mathit{hkl}}\left|I_{\mathit{hkl}}^{\mathit{even}},-,I_{\mathit{hkl}}^{\mathit{odd}}\right|}{\frac{1}{2}\left|I_{\mathit{hkl}}^{\mathit{even}},-,I_{\mathit{hkl}}^{\mathit{odd}}\right|}$.

^bR_work = Σ||F_obs| − |F_calc||/Σ|F_obs|, where F_obs and F_calc are the observed and calculated structure-factor amplitudes respectively.

^cR_free was calculated as R_work using a randomly selected subset of unique reflections not used for structure refinement.