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. 2021 Jun 23;12(7):641. doi: 10.1038/s41419-021-03924-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Serial measurement of serum HBV DNA levels in C57BL/6 mice after induction of infection with HBV 1.0 mers. Data are represented as mean ± s.e.m. from three independent experiments (n = 15 for genotype A2, n = 12 for genotype D3, n = 15 for genotype C2). Statistical analyses using unpaired two-tailed t tests were performed comparing each week with week 1. *P < 0.05, **P < 0.01. B Quantitative analysis of HBsAg in serum of C57BL/6 mice at indicated time points after induction of infection with HBV 1.0 mers. Data are represented as mean ± s.e.m. from two independent experiments (n = 12 for genotype A2, n = 11 for genotype D3). C Quantitative analysis of HBeAg in serum of C57BL/6 mice at indicated timepoints after induction of infection with HBV 1.0 mers of indicated genotypes. Data are represented as mean ± s.e.m. from two independent experiments (n = 12 for genotype A2, n = 11 for genotype D3). nd = non-detectable. D Immunohistochemistry staining of HBV core antigen positive cells in liver sections of C57BL/6 mice one week after induction of infection with the indicated HBV genotypes, arrows indicate HBcAg-positive stained liver cells, n = 6 per group, scale bars: 125 μm. Data is representative of two independent experiments. E Northern blot analysis of total liver HBV RNA from C57BL/6 mice at indicated time points after induction of HBV infection with indicated genotypes. Data is representative of two independent experiments. F Southern blot analysis of HBV DNA extracted by Hirt-lysis from total liver of C57BL/6 mice 3 weeks post induction of infection with HBV 1.0 mer, genotype D3 (lanes 1 and 2) in comparison to mice transfected with pAAV-HBV1.2 plasmid DNA, genotype A2 (lanes 3 and 4). Data is representative of two independent experiments. G Southern blot analysis of HBV DNA extracted by Hirt-lysis and exposed to heat denaturation and EcoRI digest or Exo I/III digest in comparison to untreated DNA from total liver of C57BL/6 mice 3 weeks post induction of infection with HBV 1.0 mer (genotype A2 or D3). Results are representative of three independent experiments. H Quantification of HBV cccDNA from total liver of C57BL/6 mice 1- and 5-weeks post induction of infection with HBV 1.0 mer (genotype A2 and D3) and treatment with specified compounds. Data are represented as mean (n = 3 per time point). HBV cccDNA levels expressed as a ratio of cccDNA copies per genomic equivalents (GEq). The limit of detection is 1 cccDNA copy/2 mg of liver.