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. 2021 Jun 23;11:13163. doi: 10.1038/s41598-021-89451-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Global miRNA-seq analysis of MyHC-mutant, TnT-mutant mouse hearts and human-HCM. (a,b) Volcano plots of normalized miRNA reads in hearts of (a) MyHC-mutant mouse hearts, (b) TnT-mutant mouse hearts and littermate-control hearts (ContM and ContT). Each dot represents individual miRNA. The values are means of three biological replicates. Significantly (adjusted p < 0.05) upregulated miRNAs (miR-99b-5p, miR-195a-5p, miR-497a-5p) are shown in magenta and significantly downregulated miRNA (miR-150-5p) is shown in cyan in (b). miRNAs with > 300 normalized reads in any of the genotypes are shown. (c,d) Volcano plot of log2(fold-change) of miRNA-seq results in (c) MyHC-mutants and (d) TnT-mutants compared to littermate-controls. Benjamini–Hochberg method was used to adjust p values for multiple comparisons (n = 3 biological replicates). (e,f) Heatmap of log2(fold-change) of normalized miRNA reads in MyHC-mutants and TnT-mutants compared with littermate-controls (means of three biological replicates are presented). miRNAs are sorted in descending order of fold-change in abundance in (e) MyHC-mutants and (f) TnT-mutants. (g) Normalized miRNA abundance of miR-150-5p, miR-99b-5p, miR-195a-5p, and miR-497a-5p (mean ± S.D. n = 3 biological replicates; *p < 0.05 using 2-sided unpaired Student’s t-test). (h) Volcano plots of normalized miRNA reads in human myectomy tissue and control-hearts. (i) Heatmap of log2(fold-change) of normalized miRNA reads in human myectomy tissue (n = 107) compared to control-hearts (n = 20). miRNAs are ordered in descending order of fold-change in abundance (means are presented). (j) Normalized miRNA abundance of miR-150-5p, miR-99b-5p and miR-195a-5p (mean ± S.D.; n = 107 HCM patients/n = 20 controls; *p < 0.05 using 2-sided unpaired Student’s t-test).