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. 2021 Jun 23;11:13159. doi: 10.1038/s41598-021-92264-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of NAFLD phenotype in 2-chamber systems. Oil Red O staining showing lipid accumulation in hepatocytes after 14 days in recirculating 2-chamber systems containing (a) Healthy BMM (b) Diabetic BMM (c) Diabetic + obese BMM (d) Proinflammatory BMM (e) Diabetic + proinflammatory BMM (f) Diabetic, obese + proinflammatory BMM). (g) Quantification of hepatocyte lipid accumulation. ICC quantification of insulin resistance biomarkers after 14d in recirculating (h) Healthy BMM (i) Diabetic BMM (j) Diabetic + Obese BMM (k) Diabetic, obese, and proinflammatory BMM. Statistical analysis performed as multi-way ANOVA followed by Dunnett’s test (α = 0.05) using the coverslip-wide average of two independent coverslips per condition.