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. 2021 Jun 23;11:13159. doi: 10.1038/s41598-021-92264-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Oil Red O staining showing lipid accumulation in hepatocytes after 14 days in recirculating diabetic, obese, proinflammatory BMM with (a) Control (b) 30 μM Metformin. (c) Quantification of effect of metformin for each medium condition. Oil Red O staining showing lipid accumulation in hepatocytes after 7 days in recirculating diabetic, obese, proinflammatory BMM with (d) Control (e) 1 mM Metformin. (f) Quantification of the effect of metformin for each medium condition. Statistical analysis was performed with a one-way ANOVA followed by Dunnet’s test with the relative change in each condition to block for TNF-α addition and lipid addition (α = 0.05) using between 2 and 4 independent coverslips (e) or 3 independent coverslips per condition (f).