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. 2021 Jun 23;12(7):642. doi: 10.1038/s41419-021-03930-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A RNA-seq of DNMT1, DNMT3a, and DNMT3b in RSC96 cells treated with normal glucose and high glucose. B Western blot and statistical analysis of the effect of high glucose on DNMT1 and DNMT3a in RSC96 cells. *P < 0.05 versus N group. C Immunofluorescence of DNMT1 in RSC96 cells of normal glucose group (N) and high glucose group (H). Bar: 50 μm. D Immunofluorescence of DNMT3a in RSC96 cells of normal glucose group (N) and high glucose group (H). Bar: 50 μm. E Western blot and statistical analysis of the effect of DNMT1 and DNMT3a overexpression on TXNIP expression in RSC96 cells. *P < 0.05 versus pcDNA3.1 group. F Western blot and statistical analysis of the effect of DNMT1 and DNMT3a knockdown on TXNIP expression in RSC96 cells. *P < 0.05 versus pGenesil-1 group. G Confocal microscope observation of TXNIP expression in RSC96 cells successfully transfected with DNMT1 shRNA plasmid (pGenesil-1-DNMT1 expressing GFP tag). Oval showed those cells successfully transfected with pGenesil-1-DNMT1. Bar: 20 μm. H Confocal microscope observation of TXNIP expression in RSC96 cells successfully transfected with DNMT3a shRNA plasmid (pGenesil-1-DNMT3a expressing GFP tag). Round and oval showed those cells successfully transfected with pGenesil-1-DNMT3a. Bar: 20 μm. I Real-time PCR of TXNIP mRNA in high glucose-cultured RSC96 cells transfected with pGenesil-1, pGenesil-1-DNMT1, and pGenesil-1-DNMT3a. *P < 0.05 versus pGenesil-1 group. J Western blot and statistical analysis of the effect of MG132 and chloroquine on TXNIP expression in high glucose plus 5-Aza-treated RSC96 cells. *P < 0.05 versus 5-Aza treatment group. K Western blot and statistical analysis of the effect of MG132 and chloroquine on TXNIP expression in DNMT3a knockdown RSC96 cells. *P < 0.05 versus DMSO group.