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. 2021 Jun 23;12(7):642. doi: 10.1038/s41419-021-03930-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Western blot of phospho-Akt (Ser 473), phospho-Akt (Thr 308), and Akt and statistical analysis in RSC96 cells treated with normal glucose, high glucose and mannitol. *P < 0.05 versus N group. B Western blot and statistical analysis of DNMT1, DNMT3a, and TXNIP in RSC96 cells treated with LY294002. *P < 0.05 versus DMSO group. C Immunofluorescence of TXNIP in RSC96 cells treated with LY294002. The inset was shown with dashed square. White arrows showed positive staining. Bar: 20 μm. D Immunofluorescence of DNMT1 in RSC96 cells treated with LY294002. The inset was shown with dashed square. White arrows showed the positive staining. Bar: 20 μm. E Immunofluorescence of DNMT3a in RSC96 cells treated with LY294002. The inset was shown with dashed square. White arrows showed the positive staining. Bar: 20 μm. F Western blot and statistical analysis of the time-dependent effect of LY294002 on DNMT1, DNMT3a, and TXNIP in high glucose-cultured RSC96 cells. *P < 0.05 versus 1 h group. G Western blot and statistical analysis of the effect of insulin on DNMT1, DNMT3a, and TXNIP in RSC96 cells. *P < 0.05 versus N group, # means P < 0.05 versus H group. H Immunofluorescence of TXNIP expression in high glucose cultured RSC96 cells treated with insulin. The inset was shown with dashed square. White arrows showed the positive staining. Bar: 20 μm. I Western blot of the effect of DNMT1 and DNMT3a knockdown on TXNIP expression in LY294002-treated RSC96 cells. *P < 0.05 versus N group, #P < 0.05 versus NC group.