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. 2021 Jun 23;4:775. doi: 10.1038/s42003-021-02291-y

Fig. 6. Effects of orally administered NAC, tempol (Tem), and B12 on the parameters related to the DNA methylation and expression of the suppressor of cytokine signaling (SOCS) 1 and 3.

Fig. 6

a Liver S-adenosylmethionine (SAMe) levels. b Liver DNA methyltransferase (DNMT) 1 mRNA levels. c Liver DNMT3a mRNA levels. d Liver DNMT3b mRNA levels. e PCR products amplified with methylation (M) and unmethylation (U)-specific primer sets for the promoters of SOCS1 and SOCS3 using 50 ng bisulfite-treated liver DNA. f Liver SOCS1 mRNA levels. g Liver SOCS3 mRNA levels. HHWT, Elmo1H/H Ins2+/+; HHAkita, Elmo1H/H Ins2Akita/+. Data are expressed as means ± standard errors. The numbers of animals analyzed are given in the parentheses. mRNA levels normalized by β-actin mRNA are expressed relative to the mean levels in untreated HHWT mice as 100%. *P < 0.05 vs. untreated (Untr) HHWT mice by Tukey–Kramer Honestly Significant Differences test after one-way ANOVA; †P < 0.05 vs. untreated HHAkita mice. ‡P < 0.05 vs. B12-treated HHAkita mice.