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. 2021 Jun 23;12:3913. doi: 10.1038/s41467-021-24213-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a A scheme showing the overall strategy of this study. b t-SNE of single-cell transcriptomes of T_reg cells from healthy donor (HD) peripheral blood (PB, n = 8) and bone marrow (BM, n = 6) samples, colored by subsets. Each subset was numbered and labeled with the first letter (s) of the tissue name (P0 for cluster 0 in peripheral blood, etc.). c Projection of CCR7, HLA-DRB1, FOXP3, PTPRC and MKI67 expression onto the t-SNE plot. d Heatmaps showing the top 10 (by fold change) marker genes for each subset, excluding the ribosomal and mitochondrial genes. Scaled expression means the gene expression was centered and scaled among subsets. The fold change means the values of normalized expression of genes in a specific subset compared to the normalized expression of genes in the other subsets. e Partition graph abstraction (PAGA) analysis. Nodes represent subsets, and thicker edges indicate stronger connectedness between subsets. f Correlograms visualizing the correlation of single-cell gene expression profiles between subsets from HD PB and HD BM. g Heatmaps showing the GSVA enrichment score of T_reg cell feature pathways for each subset. h The amplified (n ≥ 2) TCR distribution of T_reg cells across different subsets, colored by TCR clonotypes. The insert pictures show the composition of unique and amplified (n ≥ 2) T_reg cell TCR clonotypes in each subset. Source data are provided as a Source Data file.