Fig. 3. Pseudotime analysis and scTCR analysis defines two differentiation paths.

a Pseudotime trajectory of T_reg cells within distinct tissues, colored by subsets. The insert picture shows the proportions of different T_reg cell paths in each cell subset (HD PB: n = 5, HD BM: n = 3). b Pseudotemporal gene-expression profiles of the top 20 (by fold change) marker genes for each path, excluding the ribosomal and mitochondrial genes. c Representative terms from Gene Ontology enrichment analysis of the differentially expressed genes for each T_reg cell path. d Heatmaps showing the GSVA enrichment score of T_reg cell feature pathways for each different path. e Pseudotemporal gene-expression profiles of suppression and proliferation-associated genes in the T_reg cell paths. f The amplified TCR distribution of T_reg cells across different paths, colored by TCR clonotypes. The insert pictures show the composition of unique and amplified (n ≥ 2) T_reg cell TCR clonotypes in each path. g The shared TCR distribution of T_reg cells between any two paths, colored by TCR clonotypes. The thickness of the black solid lines representing the relative numbers of shared TCRs. The insert pictures show the numbers of TCRs shared by two paths. h The shared TCR distribution of T_reg cells between any two subpopulations in different paths. The black lines connected two subpopulations, which contained shared TCRs. Source data are provided as a Source Data file.