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. 2021 Jun 23;12:3913. doi: 10.1038/s41467-021-24213-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Trajectory analysis for T_reg cell clusters in allo-HSCT patients, colored by subsets (nG PB: n = 3, nG BM: n = 3, aG PB: n = 4, nG BM: n = 3). The insert picture shows the proportions of different T_reg cell paths in each subset. b Heatmaps showing the GSVA enrichment score of selected pathways for each T_reg cell path. c Heatmaps showing the expression of migration and suppression-associated genes in T_reg Paths. The blue font indicated that the genes were significantly down-regulated in aGVHD patients, compared with non-aGVHD patients (p < 0.05). The red font indicated that the genes were significantly up-regulated in aGVHD patients, compared with non-aGVHD patients (p < 0.05). In c, gene normalized expressions were used and p values were determined by Wilcoxon rank-sum test. Source data are provided as a Source Data file.