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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Hepatology. 2021 Jun 15;74(1):248–263. doi: 10.1002/hep.31692

Fig. 2. SMAD7 overexpression promotes cell proliferation and activates YAP/NOTCH signaling.

Fig. 2.

(A) Effect of SMAD7 transfection on proliferation of HLE, SNU449 and MHCC97H cell lines. (B) Effect of SMAD7 transfection on apoptosis of HLE, SNU449 and MHCC97H cell lines. (C) Western blot results confirming expression of HA-tag and upregulation of YAP, SMAD7 in SMAD7-transfected cell lines. (D) Western blot results showing cytoplasmic (Cyto) and nuclear Yap expression of EGFP-transfected and SMAD7-transfected human HCC cell lines (HLE, SNU449 and MHCC-97H). β-Tubulin was used as a cytoplasmic protein loading control, HISTONE-H3 was used as a nuclear protein loading control. (E) mRNA expression of YAP and NOTCH targets (CTGF, CYR61 and JAG1). (F) Effect of SMAD7 transfection on TEAD reporter activity of HLE and SNU449 cell lines. * P <0.05, ** P <0.01, *** P <0.001.