Fig. 5.

The mTOR/p70S6K signaling pathway mediated the positive effects of CSNK2B on CRC cell proliferation. a DLD1 cells were transfected with siCSNK2B-1 and siCSNK2B-2 for 48 h, then total proteins were extracted and western blot analysis was performed to detect the levels of the indicated genes. b–c CRC cells transfected with CSNK2B-expressing plasmids or siCSNK2B-1/2 were subjected to immunoblots to verify the expression changes of the indicated genes, images (b) and densitometric quantification (c) were shown. RKO cells with stable CSNK2B overexpression were treated with rapamycin (20 nM) or DMSO, CCK-8 assays (c) and colony formation assays (d) were performed to explore whether mTOR inhibition could reverse the effects of CSNK2B overexpression on CRC cell viability. *P < 0.05, **P < 0.01