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. 2021 Jun 10;11:666597. doi: 10.3389/fonc.2021.666597

Figure 3.

Figure 3

miR-193b regulates the expression of CCND1. Either miR-193b or anti-miR-193b was used to transfect Hela and Siha cells as described. (A) Venn diagrams showed 64 significantly target genes that appeared simultaneously in miRNA target prediction algorithms TargetScan, miRDB, and miRTarBase. (B) GO analysis was performed on 64 targeted genes. (C) CCND1 expressions were measured by qRT-PCR. (D) CyclinD1 protein expression was detected using Western blot. (E) Band density of Western blot was examined using ImageJ, and protein levels were normalized to β-actin. (F) 3′-UTR luciferase reporter assay was utilized to determine the binding of miR-193b to the 3′-UTR of CCND1. Luciferase activities of WT or MUT CCND1 3′-UTR luciferase construct co-transfected with miR-193b or NC in HEK-293T cells were measured. *p < 0.05, **p < 0.01, ***p < 0.001.