Figure 4.
m6A methylation in cervical cancer. (A) The total m6A methylation level in cervical cancer tissues and adjacent normal tissues. (B) mRNA expression of METTL3, METTL14, and WTAP were measured by qRT-PCR. (C–E) Hela and Siha cells were transfected with siRNA METTL3 or negative control. METTL3 mRNA level (C), METTL3, and CCND1 protein level (D) were detected after transfected. (E) Band density of Western blot was quantified using ImageJ, and protein levels were normalized to β-actin. (F) Cellular proliferation was assessed using CCK-8 assays. (G) Flow cytometry was used to assess distributions of cells across the cell cycle. (H) The qualifications of the cell cycle were presented. *p < 0.05, **p < 0.01, ***p < 0.001.