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. 2021 Jun 10;15:617175. doi: 10.3389/fnins.2021.617175

FIGURE 5.

FIGURE 5

Rabbit retinal photoreceptors across the entire retina were damaged by photocoagulation with 100 mW laser power, 50 ms pulse duration, and 500 μm laser spot size. (A,B) Fundus photographs of extensive damage of retinal photoreceptors at 1 h and 7 days after photocoagulation. Scale bar = 2 mm. (C) Control OCT image of rabbit retina. Scale bar = 1 mm. (D) Example of OCT image of rabbit retina at 7 days after photocoagulation. Scale bar = 1 mm. Red lines in (C,D) mark retina stratification. (E,F) Histomorphology of control rabbit retina (E) and rabbit retina 7 days after photocoagulation (F). Scale bar = 100 μm. (G,H) Immunofluorescence staining of control rabbit retina (G) and rabbit retina 7 days after photocoagulation (H) with cone outer segments marker PNA, bipolar cell marker PKC-α and amacrine cell marker ChAT 7 days after photocoagulation. Scale bar = 100 μm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer.