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. 2021 Jun 10;11:643129. doi: 10.3389/fonc.2021.643129

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Copyright © 2021 Bi, An, Sun, You and Yang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RMRP is increased and promotes recruitment of DDX3X to PHGDH transcript in cisplatin-resistant ovarian cancer cells. (A) qRT-PCR was performed to analyze the RMRP expression. (B, C) cells were infected with scramble GV118 lentivirus vector (scramble) or shRNAs against RMRP (shRMRP), knockdown efficiency was confirmed by qRT-PCR (B), PHGDH expression was analyzed using Western blot (C). (D, E) RIP was performed using IgG or DDX3X antibody from parental and DDP paired SKOV3 (D) and A2780 (E) infected with scramble or shRMRP. (F, H) cisplatin-sensitive SKOV3 and A2780 cells were infected with empty pGCLV-GV166 lentivirus vector (empty) or lentivirus containing RMRP (RMRP), or RMRP, expression of RMRP was analyzed using qRT-PCR (F), PHGDH expression was investigated using Western blot (G), recruitment of DDX3X on PHGDH transcript was studied using RIP (H). *P < 0.05, n.s., not significant.