Figure 3.

Regulation of HN on CMA under oxidative stress. Under oxidative stress, Hsc70 recognizes the oxidized protein (substrate), transports it to lysosomal membrane, and binds to LAMP-2 receptor on lysosomal membrane. The endogenous HN located in lysosomal membrane cytoplasmic assay stabilizes the binding of substrate and lysosome through Hsp90, and transports the substrate to lysosomal body with the assistance of Lys-Hsc70 to remove oxidized protein. Exogenous supplement of HNG enhances the binding of substrate and lysosome mediated by Hsp90, up-regulates CMA and further reduces oxidative stress damage. HN restores the activity of cathepsin D through FPRL1, promotes autophagy and reduces the production of ROS.