Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 10;9:682944. doi: 10.3389/fcell.2021.682944

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Parisi, Knapp, Girousi, Rihs, La Scala, Schnyder, Stähli, Sculean, Bosshardt, Katsaros and Degen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Before biobanking, newly isolated cell cultures undergo a standardized quality control check. (A) In a representative set of primary cells (CLP-Lip, Forehead, Gingiva, and PDL) live imaging pictures show typical cell morphologies of the patient-derived keratinocytes (orange) and fibroblasts (green). While keratinocytes form tightly packed and cohesive colonies, fibroblasts appear with an elongated and spindle-like shape. Scale bar: 50 μm. (B) The same set of primary cells was used for qPCR (top) and immunoblot analysis (bottom) confirming E-Cadherin (CDH1) and fibronectin (FN) expression in keratinocytes (orange bars) and fibroblasts (green bars), respectively. Data are expressed as mean ± SD. n = 3. (C) Mycoplasma contamination of the new cell cultures is tested by DAPI staining of the nuclei and a PCR-based mycoplasma detection analysis. DAPI staining and PCR amplifications are shown to confirm absence of mycoplasma contamination in the same representative set of newly isolated primary cell cultures. Scale bars: 25 μm (Live Imaging keratinocytes), 50 μm (Live Imaging fibroblasts), 10 μm (DAPI). +positive control; –negative control; PDL: periodontal ligament. *p < 0.05 Ep vs. Fb. (D) Lifespan analysis of two representative keratinocyte (CLP lip, red, and forehead, orange) and two fibroblast strains (gingiva, green, and PDL, yellow) reveals that all cell cultures tested have a replicative potential of at least 35 population doublings (PD). The age of the tissue donors is indicated in the brackets. Y: year; Ep: epithelial cells; Fb: fibroblasts.