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. 2021 Jun 10;9:590655. doi: 10.3389/fcell.2021.590655

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Copyright © 2021 Wohl and Sherman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluctuation of Jurkat cell membrane in a model of the immune synapse imaged by TIRF microscopy. (A) Discrete Fourier Transform (DFT) results of fluctuations of normalized intensity of anti-CD45 antibodies labeled with Alexa647, which highlighted the plasma membrane of Jurkat cells. The cells adhered to coverslips coated with anti-CD3ε. Amplitudes of normal cells (N = 30) are in blue, adenosine triphosphate (ATP) depleted cells (N = 33) are in red and fixed cells (N = 24) are in green. (B) Frequency range of 0–3 Hz of the amplitudes in (A). Power fit parameters: a (C), b (D), sum of squared errors (SSE) (E), and Sum of powers (F) results of the amplitudes presented in (B). Error bars in panels (A–F) are SEM.