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. 2021 Jun 10;9:590655. doi: 10.3389/fcell.2021.590655

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Copyright © 2021 Wohl and Sherman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplitude spectral density (ASD) and power spectral density (PSD) analysis results of live cells diameter fluctuations before and after adenosine triphosphate (ATP) depletion. (A) Average ASD analysis results in cells before (blue line) and after (red line) ATP depletion. Control average ASD results of 20 fixed cells are also presented (green line). Specific ranges of frequencies according to their dominant mechanism of fluctuations are highlighted. N = 14 cells. (B) Focusing on ASD results in the frequencies range of 0–3 Hz out of the total frequencies range presented in image (A). R values for the power fit to the ASD results are also presented. (C) Average power fit parameter a⋅b results of the ASD analysis presented in image (B) before and after ATP depletion. (D) Average power fit parameter sum of squared errors (SSE) results of the ASD analysis presented in image (B) before and after ATP depletion. (E) Average power fit parameter “Sum of powers” results of the PSD analysis before and after ATP depletion. Error bars in (B–E) are SEM.