TABLE 1.

Populations (in percentage) of different opening states in the relaxed structures of KirBac S129R obtained through MDeNM simulations and single channel recording; comparison with KirBac3.1 WT is shown.

Opening types	KirBac3.1 WT (%)	KirBac3.1 S129R (%)
Fully open	6.8	52.8
Fully closed	50.2	0.0
Gating 124 open, gating 132 closed	28.8	0.0
Gating 132 open, gating 124 closed	14.2	0.0
Gating 129 closed	0.0	47.2
Current recordings of KirBac3.1 in planar lipid bilayers	9.9 (±1.3, n = 1803) (ref 14)	44.05 (± 2.6% mean ± S.D. n= 16,107)