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. 2021 Jun 10;12:674047. doi: 10.3389/fmicb.2021.674047

TABLE 1.

List of primers used in PCR and nested PCR in this study.

Source Primers Expected size Reference
Human Martellini et al., 2005
Single PCR
Humito2-G 5′AGCCCTTCTAAACGCTAATCCAAGCCT-3′ 659
Humito2-D 5′-CTTGTCAGGGAGGTAGCGATGAGA-3′
Nested PCR
Humito11-G 5′-CCACTACTAGGCCTCCTCCTA-3′ 612
Humito11-D 5′-TAGCGATGAGAGTAATAGATAGGG-3′
Cat This study
Single PCR
CatMito1-F 5′-CCTGTCCACACTACTTGTACTCATCGC-3′ 539
CatMito1-R 5′-AGATGGTTGTTTAGGATGGCTACG-3′
Nested PCR
CatMito2-F 5′-ATTTGATCCTATAGGGTCCGCC-3′ 350
CatMito2-R 5′-CCTATGAGCGACATGATGAAAGC-3′
Dog This study
Single PCR
DogMito1-F 5′-ATGGCTCTAGCCGTTCGATTAAC-3′ 638
DogMito1-R 5′-GGCTAGGAGGACTGAGGTGTTGAG-3′
Nested PCR
DogMito2-F 5′-CATTAGGATTCACAACCAACCTGTTA-3 236
DogMito2-R 5′-AATAATGCCGGTAGGAGGTCAG-3′