Figure 6.

Effects of the [M₂L₃]⁴⁺ (M=Ru, Ni) cylinders on SARS‐CoV‐2 infection of Vero cells. Cells were infected with SARS‐CoV‐2 (MOI=0.04) in the presence or absence of cylinders and fixed at 48 hours post‐infection and spike‐protein expression quantified by rabbit anti‐spike‐protein monoclonal antibody (CR3022) and mouse anti‐rabbit Alexa 555 (green). Cell nuclei were visualised with Hoechst 33342 (blue). Total cell numbers and percentage of spike‐protein‐expressing cells were enumerated by high content imaging at x10 magnification using a CellInsight CX5 high content microscope (Thermo Fisher Scientific). A) Representative images of untreated or 75 μm [Ni₂L₃]⁴⁺ or [Ru₂L₃]⁴⁺ treated cells. B) Data represents the mean from three independent experiments and the error bars show standard deviations. Statistical analyses show Student's t tests with Welch's correction compared to no cylinder (dotted line), * p=0.0168 and ** p=0.0037.