Figure 6.

NNT-AS1 is a sponge of miR-496. (A) The predicted miR-496 binding sites on wt or mut NNT-AS1. (B) CRC cells were transfected as indicated, and the expression of miR-496 was measured via RT-qPCR. (C) A luciferase reporter assay was performed to measure the luciferase activity in CRC cells after co-transfection of miRNA mimics and NNT-AS1 wt or mut. (D) CRC cells were transfected as indicated, and the expression of NNT-AS1 was measured via RT-qPCR. (E) CRC cells were transfected as indicated and the expression of miR-496 was measured. (F) CRC cells were transfected as indicated and cell viability was measured by the MTT assay. (G) Cell migration was measured. (H) Cell invasion was measured. (I) The levels of miR-496 in CRC were measured. (J) CRC cells were transfected as indicated and the migration and invasion of cells were measured. (K) CRC cells were transfected as indicated and cell viability was measured. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-NC mimics or as indicated. All experiments were conducted at least three times. NNT-AS1, lncRNA nicotinamide nucleotide transhydrogenase antisense RNA 1; CRC, colorectal cancer; miR, microRNA; NC, negative control; si-, small interfering RNA; wt, wild-type; mut, mutant; pcD, overexpression vector; RT-qPCR, reverse transcription-quantitative PCR.