Figure 7.

RAP2C is a direct target of miR-496. (A) The constructed luciferase reporter plasmids containing the predicted wt or mut miR-496 binding sites on RAP2C. (B) A luciferase reporter assay was performed to measure the luciferase activity in CRC cells after co-transfection of miRNA mimics and RAP2C wt or mut. (C) CRC cells were transfected with miR-496 mimics, si-NNT-ASI or pcDNA.NNT-ASI and the levels of the indicated proteins were measured by western blotting. (D) RAP2C was overexpressed in CRC cells by transfection with pcDNA.RAP2C. (E) CRC cells were transfected as indicated and cell viability was measured by an MTT assay. CRC cells were transfected as indicated and (F) migration and (G) invasion were measured. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. miR-NC mimics or as indicated. All experiments were conducted at least three times. RAP2C, Ras-related protein Rap-2c; miR, microRNA; wt, wild-type; mut, mutant; NC, negative control; si-, small interfering RNA; pcDNA, overexpression vector; CRC, colorectal cancer; NNT-AS1, lncRNA nicotinamide nucleotide transhydrogenase antisense RNA 1.