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. 2021 Jun 10;12:667065. doi: 10.3389/fphys.2021.667065

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Copyright © 2021 Ronzhina, Stracina, Lacinova, Ondacova, Pavlovicova, Marsanova, Smisek, Janousek, Fialova, Kolarova, Novakova and Provaznik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of patch-clamp analysis of sodium channels in differentiated NG108-15 cells: (A) representative examples of current traces recorded after 30 min long incubation of cells in the presence of 2 μM di-4-ANEPPS (stained) or under the control conditions (non-stained); (B) averaged current-voltage relations for sodium current recorded after 30 min long incubation of cells in the presence of 2 μM di-4-ANEPPS (stained, n = 31) or under the control conditions (non-stained, n = 27); **p < 0.01; ***p < 0.001 (ANOVA and Tukey test); (C) averaged current-voltage relations for sodium current recorded after 30 min long incubation of cells in the presence of dye-free DMSO in the dilution 1:1,000 (DMSO, n = 19) or under the control conditions (non-stained, n = 16). In panels (B,C), solid lines represent B-spline fit to the experimental data.