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. 2021 Jun 10;12:667065. doi: 10.3389/fphys.2021.667065

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Copyright © 2021 Ronzhina, Stracina, Lacinova, Ondacova, Pavlovicova, Marsanova, Smisek, Janousek, Fialova, Kolarova, Novakova and Provaznik.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Electrograms (EG) recorded before the onset (0′) and during 10-min ischemia in the stained (left) and the non-stained (right) rabbit isolated heart. In the stained heart EG, ischemia manifestations (inverted, increased and widened QRS, elevated/depressed ST, inverted T wave) appear in the 8th–9th minute, whereas in non-stained heart, the first ischemia-induced changes are obvious in EG from the 2nd–3rd minute. As a result, morphology of EG recorded in the stained and the non-stained heart significantly differs at the beginning and middle of ischemia and become similar at the end of the ischemia.