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. 2021 Jun 24;143:116377. doi: 10.1016/j.trac.2021.116377

Table 1.

The system ratio γ and the corresponding limit of detection of different devices.

Category Characteristics Thermal cycling time (min) Reactor size (μL) System ratio γ (min/μL) Limit of detection Reference
γ ≥ 5.88 Low compatibility with clinical diagnosis of infectious diseases ~ 2.16 ~ 0.02 108 75 copies/single well [19]
6 0.1 60 NA [43]
26.5 1.3 20.38 0.1 ng/μL [49]
25.5 1 25.5 12 ag (~2 copy number) [39]
50 8.5 5.88 80 ng/μL [44]
γ < 5.88 High compatibility with clinical diagnosis of infectious diseases 24 20 1.2 1 pg [5]
10 10 1 1 ng/μL [47]
30 40 0.75 1.0 TCID50/mL [36]
20 25 0.8 10 copies [38]
7.5 10 0.75 1 pg/μL [48]
30 40 0.75 1.0 TCID50/mL [51]
30 40 0.75 1.0 TCID50/mL [21]
6.14 25 0.09 125 cfu/μL [29]
13.8 50 0.276 10−3 ng/μL [40]
20 6 3.33 NA [45]
30 10 3 108 copies/μL [30]
76 25 3.04 NA [20]
50 20 2.5 NA [2]
Commercialization 25 5–30 0.83–5 200 copies/μL [46]
30 25–50 0.75 1112 copies/mL [60]
≤11 (positive)
≤30 (negative)
25–50 0.22–1.2 900 copies/mL [58]
~ 30 (positive)
≤45 (negative)
25–50 0.6–1.8 0.0200 PFU/mL [54]
≤5 (positive)
≤13 (negative)
25–50 0.1–0.52 125 genome equivalents/mL [57]
~ 45 25–50 0.9–1.8 Heat-inactivated virus ATCC VR-1986HK 500 copies/mL
Infectious virus 160 copies/mL
[53,55]