Table 1.
Category | Characteristics | Thermal cycling time (min) | Reactor size (μL) | System ratio γ (min/μL) | Limit of detection | Reference |
---|---|---|---|---|---|---|
γ ≥ 5.88 | Low compatibility with clinical diagnosis of infectious diseases | ~ 2.16 | ~ 0.02 | 108 | 75 copies/single well | [19] |
6 | 0.1 | 60 | NA | [43] | ||
26.5 | 1.3 | 20.38 | 0.1 ng/μL | [49] | ||
25.5 | 1 | 25.5 | 12 ag (~2 copy number) | [39] | ||
50 | 8.5 | 5.88 | 80 ng/μL | [44] | ||
γ < 5.88 | High compatibility with clinical diagnosis of infectious diseases | 24 | 20 | 1.2 | 1 pg | [5] |
10 | 10 | 1 | 1 ng/μL | [47] | ||
30 | 40 | 0.75 | 1.0 TCID50/mL | [36] | ||
20 | 25 | 0.8 | 10 copies | [38] | ||
7.5 | 10 | 0.75 | 1 pg/μL | [48] | ||
30 | 40 | 0.75 | 1.0 TCID50/mL | [51] | ||
30 | 40 | 0.75 | 1.0 TCID50/mL | [21] | ||
6.14 | 25 | 0.09 | 125 cfu/μL | [29] | ||
13.8 | 50 | 0.276 | 10−3 ng/μL | [40] | ||
20 | 6 | 3.33 | NA | [45] | ||
30 | 10 | 3 | 108 copies/μL | [30] | ||
76 | 25 | 3.04 | NA | [20] | ||
50 | 20 | 2.5 | NA | [2] | ||
Commercialization | 25 | 5–30 | 0.83–5 | 200 copies/μL | [46] | |
30 | 25–50 | 0.75 | 1112 copies/mL | [60] | ||
≤11 (positive) ≤30 (negative) |
25–50 | 0.22–1.2 | 900 copies/mL | [58] | ||
~ 30 (positive) ≤45 (negative) |
25–50 | 0.6–1.8 | 0.0200 PFU/mL | [54] | ||
≤5 (positive) ≤13 (negative) |
25–50 | 0.1–0.52 | 125 genome equivalents/mL | [57] | ||
~ 45 | 25–50 | 0.9–1.8 | Heat-inactivated virus ATCC VR-1986HK 500 copies/mL Infectious virus 160 copies/mL |
[53,55] |