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. 2021 Jun 9;12:669213. doi: 10.3389/fphar.2021.669213

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Copyright © 2021 Tian, Zeng, Zhao and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Angelicin protectsM1 polarization and M2 macrophage polarization in vitro. (A) Schematic diagram of bone marrow macrophages (BMMs) isolation and macrophage M1/M2 polarization and repolarization strategy. (B) Representative morphology images in each group of three independent experiments. Bar represents 10 μm. (C, D) Immunostaining of enzyme-inducible nitric oxide synthase (iNOS) and CD206 (red) in isolated BMMs in different groups with quantification of iNOS⁺ and CD206⁺ cell numbers. (E, F) Western blot assay and qRT-PCR assay of Arg-1, iNOS and β-actin in BMMs. (G) qRT-PCR assay of IL-1β and TNF-α expression in BMMs. (H) ELISA for IL-1β and TNF-α concentration from cellular supernatant. The data in figures represents the mean ± SD. Significant differences are indicated as **p < 0.01. Scale bar represents 30 μm. DAPI, 4′,6-diamidino-2-phenylindole; IL, interleukin; LPS/IFN-γ, lipopolysaccharide/interferon-γ; ANG, angelicin.