KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal IgG anti-NMIIB	Cell Signaling Technology	3404S; RRID: AB_1264210
Rabbit monoclonal IgG anti-NMIIB	Cell Signaling Technology	8824S; RRID: AB_11217639
Rabbit polyclonal IgG anti-NMIIA	BioLegend	P909801; RRID: AB_2565100
Goat anti-rabbit IgG AlexaFluor 488	Thermo Fisher Scientific	A-11008; RRID: AB_143165
Goat anti-rabbit IgG AlexaFluor 568	Thermo Fisher Scientific	A-11011; RRID: AB_143157
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
NucLight Rapid Red	Essen Biosciences	4717
Blebbistatin	Sigma	B0560
Fugene 6	Promega	E-2691
Lipofectamin 2000	Thermo Fisher Scientific	11668027
AlexaFluor 488 Phalloidin	Thermo Fisher Scientific	A12379
AlexaFluor 568 Phalloidin	Thermo Fisher Scientific	A12380
Critical Commercial Assays
QIAprep spin Miniprep Kit 250	QIAGEN	27106
Experimental Models: Cell Lines
HeLa	American Type Culture Collection	CCL-2
Cos-7	Gift from Jeniffer Lipincott Shwartz	N/A
M2	Gift from Matthew Tyska	N/A
H9	WiCell Research Institute	WA09
HAP1 Parental	Horizon Discovery	C631
HAP1 myh9 KO	Horizon Discovery	HZGHC00108
Oligonucleotides
SmartPool siRNA against myh9	GE Dharmacon	E-007668-00-0005
SmartPool siRNA against myh10	GE Dharmacon	E-023017-00-0010
Individual siRNA against myh9	GE Dharmacon	A-007668-13-0005
Individual siRNA against myh10	GE Dharmacon	A-023017-15-0005
Recombinant DNA
siRNA resistant NMIIB (N-terminal) mEGFP	This study	N/A
siRNA resistant NMIIB/A (N-terminal) mEGFP	This study	N/A
NMIIA/B (N-terminal) mEGFP	Gift from Miguel Vicente-Manzanarez	N/A
NMIIB/A (N-terminal) mEGFP	Gift from Miguel Vicente-Manzanarez	N/A
NMIIB (N-terminal) mEmerald	Addgene	54192
NMIIA (N-terminal) mApple	Addgene	54929
NMIIB (N-terminal) mEGFP	Addgene	35691
NMIIA (N-terminal) mEGFP	Addgene	11347
Software and Algorithms
Fiji (ImageJ)	NIH (freely available)	https://imagej.net/Fiji/Downloads
Measurement of cortex tension using micropipette aspiration MATLAB script	This study	N/A