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. 2021 Jun 24;24(7):102770. doi: 10.1016/j.isci.2021.102770

Figure 2.

Figure 2

Direct interaction between endogenous ACE2 and NHERF1 in lung and intestine cells susceptible to SARS-CoV-2 infection

(A) Constitutive expression of ACE2 and NHERF1 in HEK293T, Caco-2, Calu-3, and HK2 cells detected by western blotting (left panel). Molecular markers are indicated in kDa on the right. Relative protein expression of ACE2 and NHERF1 from the whole-cell lysate was normalized to actin and plotted in the right panel, where the highest level of ACE2 in Calu-3 (red) and NHERF1 in Caco-2 (blue) was set to 1, respectively. Shown are representative immunoblots of three experiments with similar results. Data are means ± S.D. (error bars).

(B) Visualization of ACE2/NHERF1 interactions in Calu-3 and Caco-2 cells labeled by PLA probes (red) and DAPI (blue). Representative images of confocal microscopy were shown on the left panel. Scale bar (white), 10 μm. Quantification of PLA signals per cell was graphed in the right panel (error bars are SD).