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. Author manuscript; available in PMC: 2022 May 21.
Published in final edited form as: Matrix Biol. 2021 May 21;98:49–63. doi: 10.1016/j.matbio.2021.05.004

Fig. 6. SEC analysis of dimer and trimer assembly involving γ1-LN-LEa mutants.

Fig. 6.

A. The front face of the γ1 LN domain was modified with the indicated single amino acid substitutions. The mutated γ1-LN-LEa proteins (labeled “γ1M” in graphs) were analyzed by SEC in combination with WT α1-LN-LEa and β1-LN-LEa or with β1-LN-LEa alone to determine whether trimer or dimer assembly was altered. Elution volumes of trimers (T), dimers (D) and monomers (-M-) marked in red above peaks. The mutated γ1 monomers eluted at the same volume as WT γ1 monomer. B, C. γ1-D261R produced neither dimer nor trimer. D, E. γ1-S213R produced neither dimer nor trimer. F, G. γ1-Y147R produced dimer but no trimer. H, I. γ1-R149E produced dimer but no trimer. J, K. γ1-R285E produced dimer but no trimer.